TABLE 1.
Strains and plasmids used in this study
| Bacterium or plasmid | Relevant feature(s) | Reference(s) |
|---|---|---|
| A. tumefaciens | ||
| C58 | Nopaline-agrocinopine type strain, pTiC58 | 60 |
| NTL4 | Ti plasmidless derivative, NT1 derivative; Tets | 37 |
| MLL2 | ΔexoA C58 derivative | Ramey et al., unpublished |
| TD1 | C58 derivative with pTD102 inserted; Kmr Smr Sucs | This study |
| TD2 | C58 derivative with phoR::Ω-Km; Sms Sucr; only obtained as TD2(pTD103) | This study |
| TD3 | C58 derivative with phoB::pTD104 (phoB+) | This study |
| TD5 | NTL4 derivative; pTi−phoB::pTD105 (phoB); only obtained as TD5(pTD115) | This study |
| E. coli | ||
| S17-1/λpir | λpir; Tra+, cloning host | 29, 54 |
| SY327/λpir | λpir; cloning host | 45 |
| DH5α F′ | Cloning host | 63 |
| TOP10 | Cloning host | Invitrogen |
| Plasmids | ||
| pBluescript II SK(+) | Standard cloning vector; Apr | Stratagene |
| pBBR1MCS-5 | Broad-host-range Plac expression vector; Gmr | 35 |
| pCR2.1-TOPO | TOPO TA Cloning vector; Apr Kmr | Invitrogen |
| pKNG101 | R6K ori; Sucs Smr | 30 |
| pVIK112 | R6K ori; lacZY for transcription fusions; Kmr | 29 |
| pHP45Ω-Km | Ω-Km cassette | 18 |
| pKOK6 | lacZ-Km cassette | 32 |
| pJZ383 | Ptac::gfpmut3; Spr; pVS replicon | J. Zhu; 14 |
| pBER103 | pBluescript II SK(+) carrying PtraI from A. tumefaciens R10 | This study |
| pRHG100 | pBBR1MCS-5 derivative carrying traR from A. tumefaciens R10 | This study |
| pTD102 | pKNG101 carrying phoR::Ω-Km | This study |
| pTD103 | pBBR1MCS-5 derivative carrying phoR | This study |
| pTD104 | pVIK112 carrying 5′-truncated phoB | This study |
| pTD105 | pVIK112 carrying phoB truncated at both ends | This study |
| pTD114 | pBBR1MCS-5 derivative; Plac::traR PtraI | This study |
| pTD115 | pTD114 carrying phoB | This study |
| pTD116 | pTD114 carrying lacZ-Kmr cassette from pKOK6 | This study |