TABLE 1.
Strains and plasmids
| Strain or plasmid | Relevant genotype | Source or reference |
|---|---|---|
| P. aeruginosa PA14 | Wild type | 31 |
| PA14 sadB199::Tn5B21; Tcr | This study | |
| PA14 flgK::Tn5B30; Tcr | 24 | |
| PA14 pilB::Tn5B30; Tcr | 24 | |
| PA14 fleR::TnphoA; Knr | F. Ausubel | |
| PA14 ΔrpoN | F. Ausubel | |
| PA14 crc-24::Tn5B30 | 22 | |
| PA14 gacA::kan; Knr | L. Rahme | |
| PA14 ΔlasR | F. Ausubel | |
| Plasmids | ||
| pUCP18 | Cloning vector; Cbr Apr | 35 |
| pNC5 | PA5346 in pUCP18; Cbr Apr | This study |
| pNC72 | GPS-LS construct | This study |
| pNC111 | GPS-LS construct | This study |
| pNC294 | GPS-LS construct | This study |
| pNC227 | GPS-LS construct | This study |
| pNC396 | GPS-LS construct | This study |
| pNC429 | GPS-LS construct | This study |
| pNC447 | GPS-LS construct | This study |