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. Author manuscript; available in PMC: 2016 Feb 20.
Published in final edited form as: Biochem Biophys Res Commun. 2015 Jan 19;457(4):635–639. doi: 10.1016/j.bbrc.2015.01.039

Figure 1. Glutamine decreases the phosphorylation of mTORC1 downstream targets in starved and non-starved brain endothelial bEnd.3 cells.

Figure 1

A and B) bEnd.3 cells exposed to starvation conditions for 2 hr were treated with vehicle (0 mM) or 5 mM glutamine, and lysates collected 30 min later. Glutamine administration significantly decreased the phosphorylation, but not the total levels of the mTORC1 targets A) S6K and B) S6. C and D) bEnd.3 cells under normal culture conditions were treated with vehicle (0 mM), 5 mM or 40 mM glutamine, and lysates collected 30 min later. 40 mM, but not 5 mM, glutamine treatment significantly decreased C) S6K and D) S6 phosphorylation. The total levels of these proteins did not significantly change. Data are presented as mean ± s.e.m after correction against the β-actin loading control, *p<0.05.