Fig. 3.

(A) Western blot of α-synuclein in red blood cells, liver, kidney, lung and spleen. Each lane is labeled with the name of the mouse strain with defined various genotypes as described in Table 1. +/+ refers to Snca+/+, −/− refers to Snca−/−, dtgA53T refers to PAC-dbTg(SNCA^A53T)+/+; Snca−/−, dtgA30P refers to PAC-dblTg(SNCA^A30P)+/+; Snca−/−. wtPAC refers to PAC-Tg(SNCA^WT)+/+; Snca−/−. (B) Table giving approximate expression levels of α-synuclein derived from western blot in Fig. 3A. The greater the number of “+” signs, the stronger the expression. A “−” sign means no signal. (C) Time course of methemoglobinemia following induction by a single i.p. injection of 4-OHPAPP(44 nmoles/kg BW) in mouse strains with defined genotypes as described in Table 1. Each measurement is the average of 5 mice. At 1 h, methemoglobinemia was elevated more than two-fold in Snca−/− versus Snca+/+ (**p = 0.0011, one-tailed Mann–Whitney test) and showed a barely significant decrease in A53T versus Snca+/+ (*p = 0.024, one-tailed Mann–Whitney test). At 2 h, methemoglobinemia was prolonged 5–7 fold in Snca−/− and wtPAC lines versus Snca+/+ mice (**p = 0.0022, *p = 0.016, respectively, one-tailed Mann–Whitney test). All lines recovered equally by 4 h.