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Images from SCLM of a 7-day P. aeruginosa biofilm stained with the double-stranded DNA stain PicoGreen. (A) Reconstructed three-dimensional image of a mushroom-like structure. Bar, 50 μm. (B) Horizontal section of the biofilm at the glass surface showing that the material between cells is stained as well as the cells in the biofilm. Bar, 10 μm.
