TABLE 1.
Strains and plasmids used for this study
| Strain or plasmid | Characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| P. aeruginosa | ||
| PAO1 | Parent | B. Iglewski |
| Δ1383Sm | PA1383 mutant of PAO1, Smr Spr | This study |
| ΔpslAGm | PA2231 mutant of PAO1, Gmr | This study |
| ΔpslAnull | PA2231 mutant of PAO1, unmarked | This study |
| Δ3552Gm | PA3552 mutant of PAO1, Gmr | This study |
| E. coli | ||
| DH5-α | 24 | |
| S17-1λpir | thi pro hsdR recA RP4-2 (Tet::Mu)(Km::Tn7) λ | 21 |
| SM10 | thi-1 thr leu tonA lacY supE recA::RP4-2-Tc::Mu Kmr | 28 |
| Plasmids | ||
| pKV69 | Suicide vector, Cmr Tcr | 3 |
| pGmΩ1 | Source of Ω (aacC1 with transcriptional termination signals in both orientations), Ampr | 27 |
| pHRP315 | Source of Ωf (aadA with transcriptional termination signals in both orientations), Smr Spr | 20 |
| pEX18Tc | Suicide vector, Tcr | 11 |
| pMRP9-1 | GFP expression vector, Cbr | 6 |
| Mini-CTX-lacZ | Suicide vector, Tcr | 2 |
| pMM11 | pKV69 carrying 4.7-kb (from bp 1500183 to 1505052) fragment containing PA1383, in BamHI-SphI site | This study |
| pMM12 | pMM1 with aadA Smr Spr (SmaI-EcoRV fragment from pHRP315) cassette between the two BstEII sites of ORF PA1383 | This study |
| pMM21 | pKV69 carrying 4.4-kb (from bp 24521491 to 2456529) fragment containing PA2231, in BamHI-PstI site | This study |
| pMM22 | pMM2 with aacC1 Gmr cassette (SmaI fragment from pGmΩ1) between the two MluI sites of ORF PA2231 | This study |
| pMM31 | pEX18Tc carrying 1.2-kb (from bp 3978642 to 3979870) fragment of PAO1 chromosomal DNA in HindIII- BamHI site | This study |
| pMM32 | pMM3 with 1.2-kb (from bp 3981000 to 3982279) fragment of PAO1 chromosomal DNA in BamHI-EcoRI site | This study |
| pMM33 | pMM31 with aacC1 Gmr cassette (BamHI fragment from pGmΩ1) in BamHI site | This study |
| pMMC2231 | Mini-CTX-lacZ carrying 1.9-kb (from bp 2453177 to 2455101) fragment of PAO1 chromosomal DNA in BamHI-SphI site | This study |