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. 2015 Mar 16;112(13):4086–4091. doi: 10.1073/pnas.1417273112

Fig. 3.

Fig. 3.

ANP inhibits LPS-regulated E-selectin–dependent adhesion of cancer cells to vascular endothelial cells. (A) Representative images of the adhesion of tumor cells (A549-EGFP, Upper; H460-EGFP, Lower) to monolayer-cultured HPAECs pretreated with or without ANP. (B and C) Bar graphs showing the number of A549-EGFP cells (B) or H460-EGFP cells (C) attached to monolayer-cultured HPAECs pretreated with or without ANP. Data are means ± SEM (n = 3, each group). *P < 0.05, **P < 0.01, ***P < 0.001, unpaired two-tailed t test. (D) Representative images of A549-EGFP cells attached to HPAECs depleted of the indicated molecules by siRNA treatment and treated with LPS. (E) Bar graph showing the number of A549-EGFP cells attached to HPAECs treated as in (D). Data are means ± SEM (n = 4–5, each group). *P < 0.05, one-way ANOVA. (Scale bars, 500 μm.)