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. 2015 Mar 12;112(13):3985–3990. doi: 10.1073/pnas.1503152112

Fig. 3.

Fig. 3.

STAT3 Y705 phosphorylation is required for K49 dimethylation of STAT3. (A) WT-STAT3 cells were treated with IL-6 (50 ng/mL) and sIL-6R (62.5 ng/mL) for 1 or 4 h, respectively, and total cell lysates were analyzed for K49 dimethylation by using an antibody that recognizes this specific modification. (B) A4 cells expressing WT or K49R STAT3 were treated with IL-6 (50 ng/mL) and sIL-6R (62.5 ng/mL) for 1 h, and total cell lysates were analyzed for K49 dimethylation. (C) A4 cells expressing WT or Y705F STAT3 were treated with IL-6 (50 ng/mL) and sIL-6R (62.5 ng/mL) for 1 h, and total cell lysates were analyzed for K49 dimethylation and Y705 phosphorylation. (D) hTERT-HME1 cells were treated with OSM (100 ng/mL) or IL-6 (50 ng/mL) and sIL-6R (62.5 ng/mL) for 1 h, and total cell lysates were analyzed for K49 dimethylation, Y705 phosphorylation, and total STAT3.