TABLE 1.
Strains of P. aeruginosa and E. coli and plasmids used
| Bacterial strain or plasmid | Genotype or phenotype | Reference or source |
|---|---|---|
| P. aeruginosa | ||
| PAO1 | Wild type | 15 |
| PA6261 | PAO1 Δanr | 43 |
| RM536 | PAO1 dnr::tet Tcr | 3 |
| PAO9104 | PAO1 narL::cat Cmr | 23 |
| CHA-A2 | CHA ihfA::tet Tcr | 13 |
| PAO-MW20 | PAO1 rpoS::aacC1 Gmr | 39 |
| PAO6281 | PAO1 gacA::Ω-Sp/Sm | 30 |
| PAO-ME1 | PAO1 Δpta::aacC1-gfp Gmr | This study |
| PAO-ME3 | PAO1 ΔldhA::aacC1-gfp Gmr | This study |
| E. coli | ||
| DH5α | DH5a supE44 ΔlacU169 (φ80 lacZΔM15) hsdR recA1 endA1 gyrA96 thi-1 relA1 | 20 |
| SM10 | thi-1 thr leu tonA lacY supE recA::RP4-2-Tc::Mu Kmr | 12 |
| Plasmids | ||
| pEX18Ap | Apr; oriT+sacB+; gene replacement vector with MCS from pUC18 | 22 |
| pQF50 | Apr; promoterless lacZ gene | 17 |
| pPS858 | Apr, Gmr; source of gentamicin cassette | 22 |
| pMEP1 | Apr; pEX18Ap with a BamHI-HindIII-digested PCR fragment (2,139 bp) of the pta gene | This study |
| pMEP2 | Apr, Gmr; pMEP1 with 288-bp SalI deletion in the pta gene replaced with Gmr-gfp fragment from pPS858 | This study |
| pMEP5 | Apr; pEX18Ap with a BamHI-HindIII-digested PCR fragment (1,865 bp) carrying the 3′ end of gacS, complete ldhA, and the sequence downstream of ldhA | This study |
| pMEP11 | Apr, Gmr; pMEP5 with 727-bp SdaI deletion in the ldhA gene replaced with Gmr-gfp fragment from pPS858 | This study |
| pACKA | pQF50 containing a 291-bp fragment of the putative promoter region of loci ackA-pta | This study |