Fig. 4.

Proteinase K digestion analysis of Cpn60/Cpn20-assisted refolding of CF₁γ. (A) Schematic representation of molecular interactions during the experiment. The Cpn60 and unfolded CF₁γ were incubated in the absence (Left) or presence (Right) of Cpn20 and ADP for 10 min. Following these steps, the protein mixtures were treated with proteinase K for 0–20 min. (B) Immunoblot analysis of CF₁γ after proteinase K treatments corresponding to the procedure described in A, Left. The proteins were separated by 15% (wt/vol) SDS/PAGE, and the Cpn60 degradation product can be detected on 7% (wt/vol) SDS/PAGE. (C) Immunoblot analysis of digestion of CF₁γ by proteinase K corresponding to the procedure described in A, Right. The experimental analysis was the same as in B.