Fig. 2.

OB morphology and TFA contents in WT, vps29, and sdp1 mutants. (A–L) Detection of OBs in WT and mutant (vps29, sdp1) seeds and hypocotyl seedlings during postgerminative growth on MS medium without sucrose. Nile Red staining of OBs and confocal microscopy observation are shown for WT (A, D, G, and J), vps29 (B, E, H, and K), and sdp1 (C, F, I, and L). At least three independent experiments were performed for each kinetic point, and >10 seeds or seedlings were analyzed per experiment. (Scale bars: 5 µm.) (M) Quantification of C20:1 in dry seeds (0 DAG) and seedlings (2, 3, and 4 DAG) grown on MS medium with sucrose for WT, vps29, and sdp1.4 mutants. Level of C20:1 is expressed in nmol per seed or seedling. Experiments were repeated twice on four to six samples of 20 dry seeds or 20 seedlings per germination time. Values are mean ± SEM.