Fig. 3.

SDP1 subcellular localization during postgerminative growth. (A–D) WT and vps29 seedlings stably expressing GFP–SDP1 under the 35S promoter were grown on MS medium with 1% (wt/vol) sucrose. At 4, 5, or 8 DAG, seedlings were incubated in Nile Red and imaged by confocal microscopy to visualize OBs and SDP1 in hypocotyl cells. At 4 DAG, GFP–SDP1-labeled compartments (in green) are distinct from OBs (in red) in WT (A) and vps29 (B) hypocotyls. At 5 DAG for WT (C) and 8 DAG for vps29 (D), GFP–SDP1 labeling is found around the OBs. White arrowhead shows the string-like structure. For each time point, three independent experiments were performed, and at least five seedlings were analyzed per experiment. (E–G) Identification of SDP1-labeled compartments at young developmental stages. At 4 DAG, WT (E and G) or vps29 (F) seedlings grown on MS medium with 1% (wt/vol) sucrose were incubated in Nile Red, and colocalization of GFP–SDP1 (in green), mCherry–PTS1 (peroxisome marker in gray or blue), and Nile Red (in red) was detected by confocal microscopy. Confocal sections in G show that SDP1-labeled tubular extensions colocalized with the peroxisome marker. At least 10 WT and vps29 seedlings were analyzed. (Scale bars: 5 µm.)