FIG. 4.

Protease susceptibility of the purified YscC oligomer. Purified YscC oligomers were incubated with 2% SDS for 10 min at room temperature for analysis of the native form or at 100°C for analysis of the nonnative form. The oligomers were then incubated with the indicated concentrations of proteinase K for 10 min at room temperature. Samples were loaded on a 3 to 9% polyacrylamide gradient gel. The position of the YscC oligomer to indicated on the right of the gels. Proteins were visualized by staining with silver.