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Pore activity of the YscC oligomer. (A) Channel recording of multiple insertions of the purified YscC oligomer in a planar lipid bilayer at a membrane potential of 100 mV. The sizes of the transitions are indicated. (B) Amplitude histogram of channel openings (n = 204) at 100 mV. (C) Voltage-ramp analysis of multiple YscC channels from 0 to 200 mV and 0 to −200 mV over a total time span of 200 s.
