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. 2015 Mar 17;112(13):3991–3996. doi: 10.1073/pnas.1416163112

Fig. 4.

Fig. 4.

Φ-O-Me-cAMP binds Epac, without activation. (A) CFP signal increase after YFP photobleach in HEK293 cells. (B) Extent of YFP photobleach (n = 75) and baseline FRET efficiency of CFP-ICUE1-YFP (n = 85). (C) Mean ICUE1 signal at baseline (n = 45 and 87), upon 10 μM OMe-CPT (n = 58), or 10 μM Φ-O-Me-cAMP (n = 91) in HEK293 cells after 30-min incubation. (D) Epac1-YFP translocation in cultured cardiomyocytes from WT at baseline (n = 27), with Φ-O-Me-cAMP ± OMe-CPT (n = 31 and n = 17) and with OMe-CPT alone (n = 27). (E) Ca spark frequency (CaSpF) in myocyte under the same treatments (n = 18, n = 13, n = 13, and n = 7, respectively). (F) HDAC5-GFP translocation in cultured myocytes from WT at baseline (n = 64), with OMe-CPT (n = 19), and plus increasing [Φ-O-Me-cAMP] (n = 13, 10, 8, 9, 10). *P < 0.05 vs. CTL.