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14-3-3 proteins protect Cdt2 phosphorylated at T464 from degradation. (A) 293T cells were transfected with Flag-Cdt2 containing phosphomimetic or nonphosphorylatable residues at position 464 as indicated and treated with cycloheximide (80 μg/ml) for different times. The cell lysates were probed with anti-Flag antibodies. (B) 293T cells were transfected with plasmids as indicated. The procedure was otherwise as described in the legend to Fig. 4D. Bottom, 0.5% of the input lysates.
