FIG 8.

Preventing the accumulation of Set8 partly rescues the G₂/M arrest seen when 14-3-3γ is depleted. (A) Half-life measurement of Set8 protein in siRNA-treated HeLa cells using cycloheximide (100 μg/ml) for different times. The lysates were analyzed by Western blotting with indicated antibodies. (B and C) FACS analysis of different cell cycle phases in HeLa cells after treatment with the siRNAs indicated for 60 h. 4N<, cells with rereplicated DNA. (D) HeLa cells were treated with the siRNAs indicated for 60 h. The relative numbers of viable cells are plotted as the averages of the results of the three independent experiments. Error bars show standard deviations. *, P < 0.05, two-tailed Student's t test. (E) HeLa cell lysates from experiments performed in parallel to those whose results are shown in panels B, C, and D were probed with the indicated antibodies.