FIG 4.

Luc-RZt species generated in the absence of Hbs1-Dom34 are associated with ribosomes. (A and B) Ribosome profiles of Δdom34 (A) and Δzuo1 Δdom34 (B) strains expressing Luc-RZ-3HA. Lysates were separated on 15 to 55% sucrose gradients and subsequently fractionated monitoring A₂₅₄. Fractions were analyzed via immunoblotting using antibodies directed against luciferase (Luc), Rps9 (40S subunit), and Rpl24 (60S subunit). The bulk of full-length Luc-RZ-3HA was recovered in the cytosolic fractions, and Luc-RZt species with a higher molecular mass were recovered in the monosome fractions, while Luc-RZt species with a lower molecular mass were recovered in the polysome fractions.