FIG 6.

The ribosomal protein Asc1 is involved in the expression of Luc-K12t. (A) Cycloheximide sensitivity of yeast is not significantly altered in the absence of Asc1 or RAC/Ssb. Strains were inoculated to the same OD₆₀₀ into medium containing the indicated concentrations of cycloheximide (CHX). Cultures were then grown until the no-CHX control had reached an OD₆₀₀ of 0.9 (100%). The OD₆₀₀ of the cultures containing CHX is given in the no-CHX control. (B) Luc-K12t expression depends on Asc1. Strains harboring the Luc-K12-3HA reporter were grown in the absence (−) or presence (+) of sublethal doses of CHX. Total extracts were analyzed on an immunoblot decorated with luciferase antibody (Luc). The positions of Luc-K12-3HA (K12-3HA) and Luc-K12t (K12t) are indicated. (C) Luc-RZt expression is independent of Asc1. Total extracts of strains harboring the Luc-RZ-3HA reporter were analyzed via immunoblotting using antibodies recognizing luciferase (Luc) and Sse1. The positions of Luc-RZ-3HA (RZ-3HA) and Luc-RZt (RZt) are indicated.