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. Author manuscript; available in PMC: 2015 Apr 6.
Published in final edited form as: Science. 2001 Apr 19;292(5520):1376–1378. doi: 10.1126/science.1060360

Fig. 3.

Fig. 3

Bud5 interacts with Axl2. (A) Bud5-HA coimmunoprecipitates with Axl2–protein C. The top and bottom panels show immunoblots carried out with monoclonal antibodies against the hemagglutinin epitope and the protein C epitope, respectively. Lanes 1 and 2 show the eluents from immunoprecipitation (IP) experiments with extracts from a and a/α cells expressing Axl2–protein C (Axl2-P.C), respectively; lanes 4 and 5 show the soluble fractions (S10) from a and a/α cells expressing Axl2–protein C, respectively. Lanes 3 and 6 show the control immunoprecipitation experiment and S10 fraction from cells expressing untagged Axl2, respectively. (B) Bud5 copurifies with the cytoplasmic domain of Axl2. The top and bottom panels show immunoblots carried out with monoclonal antibodies against the hemagglutinin epitope and with polyclonal antibodies against GST, respectively. Lane 1 shows a cells expressing GST-Axl2C, lane 2 shows a/α cells expressing GST-Axl2C, and lane 3 shows a cells expressing GST alone.