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. Author manuscript; available in PMC: 2016 May 7.
Published in final edited form as: Neuroscience. 2015 Mar 6;293:109–122. doi: 10.1016/j.neuroscience.2015.02.042

Figure 2.

Figure 2

The coordination of DSCAM and DCC in binding to TUBB3. (A) DCC shRNA or functional blocking anti-DCC antibody inhibited the induction of the interaction of endogenous DSCAM with TUBB3 by Netrin-1. E15 mouse cortical neurons were transfected with Venus YFP only, Venus-YFP plus control shRNA or Venus-YFP plus DCC shRNA and cultured for 2 d. Neurons were stimulated with Netrin-1 or the control in the presence of or absence of anti-DCC functional blocking antibody. (B) Quantification of relative binding of DSCAM and TUBB3. The y-axis shows relative binding of TUBB3 with DSCAM in arbitrary units. Data are mean ± s.e.m from three independent experiments. *** indicates p< 0.001 (One way Anova and Fisher LSD post-hoc comparisons). NS, not significant. (C) DSCAM knockdown blocked the Netrin-1-induced interaction of endogenous DCC and TUBB3. E15 mouse cortical neurons were transfected with Venus YFP only, Venus-YFP plus control shRNA or Venus-YFP plus DSCAM shRNA and stimulated with Netrin-1 or the control. Expression of DSCAM shRNA had no effects on levels of endogenous DCC and TUBB3. (D) Quantification of relative binding of DCC and TUBB3. The y-axis shows relative binding of TUBB3 with DCC in arbitrary units. Data are mean ± s.e.m from three independent experiments. *** indicates p< 0.001 (One way Anova and Fisher LSD post-hoc comparisons). NS, not significant.