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. Author manuscript; available in PMC: 2015 Apr 6.
Published in final edited form as: Ann Neurol. 2014 Jan 2;75(2):209–219. doi: 10.1002/ana.24070

Figure 5.

Figure 5

Mitochondrial reactive oxygen species ROS (mROS) production was associated with the formation of mitochondrial permeability transition pore (mPTP) protein assemblies. (A) Western blot assay for the 3 core components of the mPTP (voltage-dependent anion channel [VDAC], adenine nucleotide translocase [ANT], and cyclophilin D [CypD]) in the ipsilateral (Ipsi) versus contralateral (Contra) hemisphere at 12 hours following intracerebral hemorrhage (ICH); n=4 mice per group. (B) Western blots for VDAC in ICH without or with chemical cross-linker (disuccinimidyl suberate [DSS]) at 12 hours following operation. Monomeric VDAC is located at 30kDa. Dimeric (Di) VDAC is detected around 60kDa. (C) Quantification of ROS changes in the perihematomal region in sham, ICH, ICH+TRO-19622 (ICH+TRO), and ICH+Mito-TEMPO (ICH+MITO) groups at 12 hours following operation; n=6 mice per group. Error bars represent mean±standard error of the mean. #p<0.05 vs sham, *p<0.05 vs ICH. RFU=relative fluorescence units.