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. Author manuscript; available in PMC: 2015 May 6.
Published in final edited form as: Cell Rep. 2014 Oct 23;9(3):829–841. doi: 10.1016/j.celrep.2014.09.028

Figure 3. Development and characterization of an orthotopic EWS tumor model.

Figure 3

(A) Diagram of the injection procedure. (B-D) X-ray images of a mouse leg showing the injection procedure before, during, and after injection. (E, F) Hematoxylin and eosin staining of mouse femur and bone marrow after injection of EWS cells in Matrigel (yellow dashed line). Arrows indicates the injection site. (G) X-ray image of an orthotopic tumor with bony extensions (arrow). (H) Transverse view of the soft-tissue and bony component of the orthotopic EWS xenograft in an MR image. (I-M) Micro-PET/CT scans using 11C-methionine. The tumor (arrow) shows accumulation of the radiotracer. (N) Photograph of the femur removed from a mouse with a large mass from the orthotopic xenograft. (O, P) High- and low-power images of the orthotopic tumor showing its extension from the bone to the surrounding soft tissue.