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. Author manuscript; available in PMC: 2016 Feb 12.
Published in final edited form as: Cell Physiol Biochem. 2015 Feb 12;35(4):1317–1325. doi: 10.1159/000373953

Fig. 2.

Fig. 2

Effect of genistein or estradiol treatment on total Akt, p-Akt, p-PDK1, P-PTEN, p-GSK-3β and p-c-Raf, Rap1, and ERK1/2 protein expression in intact or ovariectomized female murine jejunum. A. Akt. Average Akt expression in female mice, following exposure to DMSO control (0G), estradiol (10E2), or genistein (600G), is shown normalized to GAPDH (n = 8–12). Representative western blot is shown above. Akt and GAPDH bands were observed at ~60 KDa and 37 KDa respectively. B. p-Akt. Average p-Akt expression in female mice, following exposure to DMSO control (0G), estradiol (10E2), or genistein (600G), is shown normalized to GAPDH (n = 9–12). Representative western blot is shown above. p-Akt and GAPDH bands were observed at ~60 KDa and 37 KDa respectively. C. p-PDK1. Average p-PDK1 expression in female mice, following exposure to DMSO control (0G), estradiol (10E2), or genistein (600G), is shown normalized to GAPDH (n = 9–11). Representative western blot is shown above. p-PDK1 and GAPDH bands were observed at ~58–68 KDa and 37 KDa respectively. D. p-PTEN. Average p-PTEN expression in female mice, following exposure to DMSO control (0G), estradiol (10E2), or genistein (600G), is shown normalized to GAPDH (n = 9–11). Representative western blot is shown above. p-PTEN and GAPDH bands were observed at ~54 KDa and 37 KDa respectively. E. p-GSK-3β. Average p-GSK-3β expression in female mice, following exposure to DMSO control (0G), estradiol (10E2), or genistein (600G), is shown normalized to GAPDH (n = 11–12). Representative western blot is shown above. p-GSK-3β and GAPDH bands were observed at ~46 KDa and 37 KDa respectively. F. p-c-Raf. Average p-c-Raf expression in female mice, following exposure to DMSO control (0G), estradiol (10E2), or genistein (600G), is shown normalized to GAPDH (n = 9–11). Representative western blot is shown above. p-c-Raf and GAPDH bands were observed at ~74 KDa and 37 KDa respectively. G. Rap1. Average Rap1 expression in female mice, following exposure to DMSO control (0G), estradiol (10E2), or genistein (600G), is shown normalized to GAPDH (n = 10–11). Representative western blot is shown above. Rap1 and GAPDH bands were observed at ~21 KDa and 37 KDa respectively. H. ERK1/2. Average ERK1/2 expression in female mice, following exposure to DMSO control (0G), estradiol (10E2), or genistein (600G), is shown normalized to GAPDH (n = 6–8). Representative western blot is shown above. ERK1/2 and GAPDH bands were observed at ~44 KDa and 37 KDa respectively.