Figure 5.

Regional delivery of Nano-taxol may replace HIPEC.

Notes: (A) Recurrent tumors were retrieved and subjected to flow cytometry analysis of the Hoechst 33342-stained side population cells. The percentage of side populations was significantly higher in the recurrent tumors (3.6%, right panel) than in the primary tumors (0.4%, left panel), indicating that the recurrent tumors harbor more cancer stem cells. Circumscribed area between red arrows indicates side population. (B) Diagram depicting the setting of HIPEC. The inflow and outflow ports and anal temperature probe used to monitor the internal temperature of the mouse during perfusion are shown. The mice were perfused for 1 hour at a rate of 3 mL per minute with Taxol^® 10 mg/kg. (C) Both the HIPEC of Taxol and the intraperitoneal delivery of Nano-taxol showed equally good control of recurrent tumor cells. (D) Survival curve and complication distribution. Both groups showed equal survival (P=0.38, log-rank test). However, more complications, including bowel obstruction or perforation, were observed in the HIPEC group. In contrast, no complications occurred in the intraperitoneal Nano-taxol group. The experiments were performed in triplicate.

Abbreviations: HIPEC, hyperthermic intraperitoneal chemotherapy; i.p., intraperitoneal; SP, side population.