Table 1.
Primer sequences
| Gene | Primer | Sequence (5′ to 3′) |
|---|---|---|
| 18S rRNA | Forward | 5′-CTCAACACGGGAAACCTCAC-3′ |
| Reverse | 5′-CGCTCCACCAACTAAGAACG-3′ | |
| Oct4 | Forward | 5′-GTGGAGAGCAACTCCGATG-3′ |
| Reverse | 5′-TGCTCCAGCTTCTCCTTCTC-3′ | |
| Sox4 | Forward | 5′-CGAGTGGAAACTTTTGTCGGA-3′ |
| Reverse | 5′-TGTGCAGCGCTCGCAG-3′ | |
| Klf4 | Forward | 5′-CCGCTCCATTACCAAGAGCT-3′ |
| Reverse | 5′-ATCGTCTTCCCCTCTTTGGC-3′ | |
| c-Myc | Forward | 5′-GGAACGAGCTAAAACGGAGCT-3′ |
| Reverse | 5′-GGCCTTTTCATTGTTTTCCAACT-3′ | |
| Nanog | Forward | 5′-ATTCAGGACAGCCCTGATTCTTC-3′ |
| Reverse | 5′-TTTTTGCGACACTCTTCTCTGC-3′ | |
| Lin28 | Forward | 5′-CCCCCCAGTGGATGTCTTT-3′ |
| Reverse | 5′-CCCTCCTTCAAGCTCCGG-3′ | |
| MDR-1 | Forward | 5′-TGGCAAAGAAATAAAGCGACTGA-3′ |
| Reverse | 5′-CAGGATGGGCTCCTGGG-3′ | |
| MRP-1 | Forward | 5′-GCTTCCTCTTGGTGATATTCG-3′ |
| Reverse | 5′-GCAGTTCAACGCATAGTGG-3′ | |
| ABCG2 | Forward | 5′-CATGTACTGGCGAAGAATATTTGGT-3′ |
| Reverse | 5′-CACGTGATTCTTCCACAAGCC-3′ | |
| E-cadherin | Forward | 5′-TGCCCAGAAAATGAAAAAGG-3′ |
| Reverse | 5′-GTGTATGTGGCAATGCGTTC-3′ | |
| Vimentin | Forward | 5′-GAGAACTTTGCCGTTGAAGC-3′ |
| Reverse | 5′-GCTTCCTGTAGGTGGCAATC-3′ | |
| Twist | Forward | 5′-GGAGTCCGCAGTCTTACGAG-3′ |
| Reverse | 5′-TCTGGAGGACCTGGTAGAGG-3′ | |
| Snail | Forward | 5′-CCTCCCTGTCAGATGAGGAC-3′ |
| Reverse | 5′-CCAGGCTGAGGTATTCCTTG-3′ | |
| HIF-1α | Forward | 5′-TTTTTCAAGCAGTAGGAATTGGA-3′ |
| Reverse | 5′-GTGATGTAGTAGCTGCATGATCG-3′ | |
| IL-8 | Forward | 5′-ATTAGCCACCATCTTACCTCACAGT-3′ |
| Reverse | 5′-ATTAGCCACCATCTTACCTCACAGT-3′ | |
| EGF | Forward | 5′-TGCCAACTGGGGGTGCACAG-3′ |
| Reverse | 5′-CTGCCCGTGGCCAGCGTGGC-3′ | |
| β-FGF | Forward | 5′-TCTTCCTGCGCATCCACC-3′ |
| Reverse | 5′-TCAGCTCTTAGCAGACATTGGAAGA-3′ | |
| PDGF-α | Forward | 5′-GAGGTGATCGAGAGGCTGG-3′ |
| Reverse | 5′-CGATAATCCGGATTCAGGCTT-3′ | |
| VEGF-α | Forward | 5′-CTTGCCTTGCTGCTCTACC-3′ |
| Reverse | 5′-CACACAGGATGGCTTGAAG-3′ | |
| CXCR4 | Forward | 5′-GGACCTGTGGCCAAGTTCTTAGTT-3′ |
| Reverse | 5′-ACTGTAGGTGCTGAAATCAACCCA-3′ | |
| CXCL12 | Forward | 5′-CTGGGCAAAGCCTAGTGA-3′ |
| Reverse | 5′-GTCCTGAGAGTCCTTTTGCG-3′ |
Abbreviations: β-FGF, beta-fibroblast growth factor; EGF, epidermal growth factor; HIF-1α, hypoxia-inducible factor-1 alpha; IL-8, interleukin-8; PDGF-α, platelet-derived growth factor-alpha; VEGF-α, vascular endothelial growth factor-alpha.