Fig 3. Relative mRNA expression levels of Ap-sHSP21.4 and immune response-related genes in response to the NPV stress by injection of siRNA at 24 and 48 h.

Expression levels were assessed using 18S rRNA gene for normalization. One set of negative control siRNAs (si-NC) was injected alongside the experimental injections. The data were analyzed by ANOVA and presented as mean ± SE of independent experiments done in triplicate and the asterisk represents the significant differences.