Table 1. Primers used in this study.
Primer | sequences (5'–3') | Application |
---|---|---|
F1 | CAACACAGCATAGCGATAGCAG | qPCR |
R1 | GATTTCTCCTCGTGTTTGGCAT | qPCR |
F18S | CGATCCGCCGACGTTACTACA | qPCR |
R18S | GTCCGGGCCTGGTGAGATTT | qPCR |
F2 | ATGGCTGACAGTGGTCTCAAG | amplification |
R2 | TCAGTGCTTCTGGATAGGAATG | amplification |
F3 | CGCGGATCC ATGGCTGACAGTGGTCTCAAG | expression |
R3 | ATAAGAATGCGGCCGCTCAGTGCTTCTGGATAGGAATG | expression |
F4 | TGCCAAACACGAGGAGA | RACE PCR |
F 5 | ACTCGCCATTACCGACAGG | RACE PCR |
R4 | TCTGCTATCGCTATGCTGT | RACE PCR |
R5 | TTTGCCGTCACCTTCATCC | RACE PCR |