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. Author manuscript; available in PMC: 2015 Apr 6.
Published in final edited form as: Leuk Lymphoma. 2014 Feb 17;55(8):1876–1883. doi: 10.3109/10428194.2013.862241

Figure 2.

Figure 2

PPP induces significant effects on downstream target proteins of IGF-IR in Jurkat and Molt-3 cells. (A) Western blotting confirmed that at 24 hours, PPP induces marked down-regulation of pIGF-IR, without a noticeable change in IGF-IR levels. In addition, PPP induces significant activation of Caspase-3 and PARP, and decreases in the levels of the antiapoptotic proteins Bcl-2 and Bcl-XL. Furthermore, PPP induces a notable increase in the cell-cycle regulatory protein cyclin B1. Changes are not seen in p16. β-actin was used as an internal control. Each experiment was repeated three times with similar results. (B) Immunoprecipitation showed that PPP induces significant down-regulation of pIGF-IR, without a noticeable change in IGF-IR levels. Representative results were shown (n=3). (C) The stimulation with IGF-1 showed that obvious down-regulation of pIGF-IR and PPP efficiently blocks IGF-IR activity and, pIGF-IR was down-regulated. β-actin was used as an internal control. Each experiment was repeated three times with similar results.