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. 2015 Jan 2;593(Pt 7):1531–1550. doi: 10.1113/jphysiol.2014.281196

Figure 1.

Figure 1

Strategy for generating and genotyping Gng13 KO mice

ES cells carrying the Gng13-null allele were obtained from KOMP. A, homologous recombination in ES cells using a bacterial artificial chromosome based construct with a Gng13 gene specific deletion and an expression selective cassette containing the LacZ reporter. The final allele lacks the entire coding region of Gng13 and part of the 3′ UTR. The positions of various primers used for genotyping are indicated. B, PCR products run on an agarose gel show the expected size products for different genotypes: TUF and TUR, 94 bp (WT and heterozygous, Het); NeoInf and NeoInR, 282 bp (KO and Het).