Table 1.
PCR primers
| Gene | Accession number | 5′ Forward position and primer | 5′ Reverse position and primer |
|---|---|---|---|
| Gng2 variant 1 | NM_010315.4 | (3372) AGCTGCTCCTGCAAATGGCGA | (3792) ACCGTTCATGTGGGGTAAAGATGCG |
| Gng3 | NM_010316.3 | (1206) AATGGCGGTCCCCAGCGTTC | (1547) GGGCTTCTTGGGTCCCAGTCC |
| Gng4 | NM_010317.2 | (2339) CCCACCCCTGCCACTCTACCA | (2749) GCCAGGCCTGTGCTGTATGG |
| Gng5 | NM_010318.2 | (177) CGCGTGAAGGTTTCCCAGGCA | (442) AGGACTGACGAGCAGAAGATAGTGT |
| Gng7 variant 1 | NM_001038655.1 | (19) TGCAGGAGAGCGGTGCCTGA | (976) GTGCATTGTGTGTGCCCCGC |
| Gng7 variant 2 | NM_010319.3 | (29) TGCGTTCGGCTGTGCGGCT | (1216) GCCACAGTCACGATGTGGGACG |
| Gng8 | NM_010320.3 | (563) TGTGACAAGCGAGACTGTGGGC | (817) CGGAAGGGATTCTCGGCGGC |
| Gng10 | NM_025277.3 | (506) GCCCTGTGTGGGATAGAGGCA | (765) TTGCCAAGGAGCAGCGCCCA |
| Gng11 | NM_025331 | (517) CCTTCAAAGAAAAGGGCAGCTGT | (785) AGTGGGGGAGCCATGCCTTCAT |
| Gng12 variant 1 | NM_001177560.1 | (3678) TGGTCAGTCCTGTGCCGGGT | (4068) TGCTGGATGGACAGCCGAAGA |
| Gng13 | NM_022422 | (143) GGAGGAGTGGGATGTGCCCCA | (403) GGACTAAAGCAGAGACACCCGGA |
| Gng5 | NM_010318.2 | (170) GCTCAACCGCGTGAAGGTTT | (259) ACTCCAGTCAGCAGAGGGTC |
| Gng10 | NM_025277.3 | (151) GGAGAGGATCAAGGTCTCCCA | (222) GCGTCCTTGCATGCATTCTG |
| Gng11 | NM_025331.2 | (407) CAAGTTGCAGAGACAACAGGTATC | (503) TGGGATTCCCTTTACCAGAGGA |
| GAPDH | NM_008084 | (5) ACGGCCGCATCTTCTTGTGCA | (85) ATACGGCCA AATCCGTTCACACCG |
Specific primers used to amplify cDNA from the ON bipolar library or for quantitative RT-PCR comparing WT and Gng13 KO retinas (last four).