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. 2015 Feb 6;593(Pt 7):1551–1580. doi: 10.1113/jphysiol.2014.280826

Figure 1.

Figure 1

Characterization of input resistance (RInput), membrane potential sag and rebound potential in dorsal and ventral pyramidal cells

A, example traces of voltage responses evoked by 1 s long current pulses (−100 pA, +50 pA) in dorsal (n = 22) and ventral (n = 25) pyramidal cells. B, summary, RInput was significantly higher for both negative and positive current pulses in ventral pyramidal cells [–100 pA: 92.3 (26.2) MΩ; +50 pA: +137.8 (40.1) MΩ; n = 25 cells; N = 17 rats] than dorsal pyramidal cells [–100 pA: 70 (17.9) MΩ; +50 pA: 107.4 (33.4) MΩ; n = 22 cells; N = 15 rats]; *P < 0.05, **P < 0.01. RInput was significantly higher during positive than negative pulses in both ventral and dorsal cells (***P < 0.001; two-way repeated measures ANOVA). C, example traces of voltage responses evoked by 1 s long negative current pulses in dorsal (n = 14) and ventral (n = 15) pyramidal cells. Injected current pulses were scaled to compare voltage responses at similar membrane potentials at the beginning of each pulse (• Peak) in dorsal and ventral pyramidal cells. D, summary, showing no significant difference in membrane potentials measured at the peak [dorsal: −81.6 (1.96) mV; n = 14 cells; N = 12 rats; ventral: −81.5 (1.94) mV; n = 15 cells; N = 12 rats; P = 0.97, two-tailed, two-sample t test after Johnson transformation] and at steady-state voltage responses (○) at the end of each pulse [dorsal: −80.1 (1.59) mV; ventral: −79.8 (1.315) mV; P = 0.64, two-tailed t test). To quantify the sag ratio, we divided steady-state voltage responses by responses measured at the peak, showing no difference between dorsal and ventral pyramidal cells [dorsal: 0.98 (0.007); ventral: 0.98 (0.014); P = 0.93, two-tailed, two-sample t test after Johnson transformation]. E, additional analysis showed no significant difference in mean rebound potentials (▪) measured after the end of each pulse in dorsal and ventral pyramidal cells [dorsal: −70.7 (0.9) mV; n = 14 cells; N = 12 rats; ventral: −70.4 (1.2) mV; n = 15 cells; N = 12 rats; P = 0.56, two-tailed, two-sample t test]. Mean (SD).