Table 2.

Properties of the mouse rod photocurrent under different experimental conditions

	D	SD1	SD2	N	SN	ZT/CT22–00	ANOVA F(4,25)	P
n	4	7	7	3	5	9
RMP	−29.9	−24.3**	−32.1	−43.9	−42.4	−27.2	7.22	0.0008
SEM	0.97	1.47	1.45	9.3	3.8	3.11
Observed Imax	10.55**	11.94**	16.59***	4.87	4.74	8.32	7.90	0.0005
SEM	1.38	1.94	1.94	1.12	0.62	1.09
Parameters obtained from fitting the absolute data to eqn 13
Imax	10.55	10.7*	16.73***	5.23	4.84	8.60	13.67	<0.0001
SEM	1.37	1.42	1.35	1.29	0.46	0.97
N	0.79	0.80	0.92	0.71	1.37	0.87	0.31	0.868
SEM	0.34	0.37	0.29	0.39	0.73	0.34
	8.93E–07	5.74E–07	4.50E–07	2.74E–06	1.63E–06	1.36E–06
SEM	5.85E–07	4.06E–07	1.92E–07	3.16E–06	6.76E–07	7.14E–07
Log()	−6.05	−6.24	−6.35	−5.56	−5.79	−5.87
SEM	−3.96	−4.41	−2.71	−6.41	−2.40	−3.08
(photons cm−2)	4.91	3.16	2.48	15.07	8.97	7.48	0.93	0.466
SEM	3.22	2.23	1.06	17.38	3.72	3.93
Fit (r2)	0.79	0.60	0.76	0.77	0.83	0.69
Fit ANOVA	F(3,20) = 57.97	F(3,35) = 48.21	F(3,35) = 102.15	F(3,15) = 30.18	F(3,25) = 81.32	F(3,45) = 77.99
P	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001
Parameters obtained from fitting the normalized data to eqn 13
Vmax (fixed)	1.00	1.00	1.00	1.00	1.00	1.00
SEM	—	—	—	—	—	—
N	0.75	0.74**	0.9*	0.86	1.49	0.92	4.66	0.0075
SEM	0.12	0.087	0.063	0.13	0.27	0.21
	9.58E–07	6.80E–07	4.78E–07	2.27E–06	1.77E–06	8.64E–07
SEM	2.19E–07	1.20E–07	4.31E–08	4.65E–07	2.48E–07	2.29E–07
Log()	−6.02	−6.17	−6.32	−5.64	−5.75	−6.06
SEM	−1.38	−1.09	−0.57	−1.16	−0.81	−1.61
(photons cm−2)	5.27**	3.74**	2.63***	12.49	9.74	4.75	14.38	<0.0001
SEM	1.20	0.66	0.24	2.56	1.36	1.26
Fit (r2)	0.93	0.93	0.98	0.96	0.96	0.77
Fit ANOVA	F(2,20) =	F(2,35) =	F(2,35) =	F(2,15) =	F(2,25) =	F(2,45) =
	342.58	613.9	2219.94	329.86	605.55	195.95
P	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001
	4.90E–05	3.65E–05	1.26E–05	7.00E–05	1.28E–05	2.13E–05
Log()	−4.31	−4.44	−4.90	−4.15	−4.89	−4.67
(photons cm−2)	270	201	69	385	70	117

Observed I_max is the value of the peak photoresponse measured with a flash of light density 440 photons μm⁻². The light density to reach 95% of saturation ( was calculated using the parameters obtained from fitting the normalized data to eqn 13. A one-way ANOVA was performed to compare the data from day (D), subjective day-1 (SD1), subjective day-2 (SD2), night (N), and subjective night (SN; right columns).

^*P < 0.05

^**P < 0.01

^***P < 0.001 when compared to respective N or SN (Tukey's post hoc test). Values collected during late N and late SN (ZT/CT22–00) are given for information but were not included in the ANOVA. RMP, resting membrane potential.