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. 2015 Mar 17;172(8):2133–2147. doi: 10.1111/bph.13036

Figure 2.

Figure 2

DIM treatment suppresses the production of systemic pro-inflammatory cytokines and apoptotic damage in livers of mice with LPS-induced ALF. Blood was collected from mice at (A) 2 and (B) 12 h after GalN + LPS (shown as LPS) injection. Sera were analysed for cytokines using sandwich elisa for TNF-α, CCL2 and IL-6. Data presented as mean ± SEM (n = 6 for the LPS + Veh group); n = 8 for the other groups). (C) Protein samples were isolated from liver tissues harvested 12 h after LPS injection and run on SDS-PAGE for immunoblotting to probe for caspase-3 (uncleaved: 35 kDa and cleaved: 17 kDa) and β-actin (42 kDa). *P < 0.05, **P < 0.01, significantly different as indicated.