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. Author manuscript; available in PMC: 2016 Apr 15.
Published in final edited form as: Vet Immunol Immunopathol. 2015 Feb 7;164(0):127–136. doi: 10.1016/j.vetimm.2015.02.001

Figure 5. Viability and effector function of canine CD4+ and CD8+ T cells measured over a 48hr period.

Figure 5

Canine blood was collected, incubated on ice, and PBMCs isolated at the indicated time points. PBMCs were incubated at 37°C with PMA/ionomycin or media alone in the presence of GolgiPlug for 5 hours. Cells were stained with anti-CD8 and anti-CD4, permeabilized, and stained for IFNγ. Numbers indicate percentage (gated) and count (bottom right) of CD8+ (A) and CD4+ (B) T cells producing IFNγ at each isolation time point.