(A) Schematic showing that there are eight consensus TAATCC DNA-binding sites in the first intron of mouse Depdc1b. The number below the diagram indicates the nucleotide residues. Three intronic regions that can be amplified by primer pairs #1, #2, and #3 are shown. “GGATTA” and “TAATCC”, the consensus TAATCC DNA-binding site. E1, the first exon; E2, the second exon. (B) ChIP assays showing the interaction of Pitx2 with the first intron of mouse Depdc1b. The results of a typical ChIP assay using primer pair #1 are shown. Similar results were obtained with primer pairs #2 and #3.