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. Author manuscript; available in PMC: 2016 Apr 10.
Published in final edited form as: Exp Cell Res. 2015 Feb 19;333(1):80–92. doi: 10.1016/j.yexcr.2015.02.008

Figure 2. Recruitment of Pitx2 to the first intron of mouse Depdc1b.

Figure 2

(A) Schematic showing that there are eight consensus TAATCC DNA-binding sites in the first intron of mouse Depdc1b. The number below the diagram indicates the nucleotide residues. Three intronic regions that can be amplified by primer pairs #1, #2, and #3 are shown. “GGATTA” and “TAATCC”, the consensus TAATCC DNA-binding site. E1, the first exon; E2, the second exon. (B) ChIP assays showing the interaction of Pitx2 with the first intron of mouse Depdc1b. The results of a typical ChIP assay using primer pair #1 are shown. Similar results were obtained with primer pairs #2 and #3.