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. Author manuscript; available in PMC: 2016 Apr 10.
Published in final edited form as: Exp Cell Res. 2015 Feb 19;333(1):80–92. doi: 10.1016/j.yexcr.2015.02.008

Figure 3. RNAi-mediated depletion of Pitx2 increases the protein levels of Depdc1b.

Figure 3

(A) C2C12 cells transfected with an siRNA against Pitx2 were subjected to immunoblot analysis with an antibody specific for Depdc1b. (B) Cell lysates from HeLa cells transfected with empty vector (lane 1) or a plasmid encoding GFP-DEPDC1B (lane 2) were subjected to immunoblot analysis with DEPDC1B antibody (top panels). (C) Cell lysates from untransfected C2C12 (lane 1), and MEF (lane 2), and NIH3T3 (lane 3) were subjected to immunoblot analysis with an antibody specific for DEPDC1B (top panels). The expected molecular mass for endogenous DEPDC1B is 61 kDa.