HeLa cells were transfected with the MYC-TRIOGEFD1 plasmid alone (a-c) or together with a plasmid encoding GFP-DEPDC1B-FL (d-g), GFP-DEPDC1B-151-529 (h-k), or GFP-DEPDC1B-1-151 (l-o). The transfected cells were subjected to immunofluorescence staining for actin filaments (a, e, i, and m) and MYC-TRIOGEFD1 (b, f, j, and n). Arrowheads indicate the transfected cells. Arrows in a, c, d, e, and g indicate the sites of cell-cell contacts. Scale bar = 20 μm.