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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Am J Obstet Gynecol. 2014 Oct 19;212(4):479.e1–479.e10. doi: 10.1016/j.ajog.2014.10.026

Figure 7.

Figure 7

Metformin increases sensitivity to paclitaxel. (A) IC50 curves for human ovarian cancer cell lines. HeyA8 and SKOV3ip1 human ovarian cancer cells were plated in complete medium and treated with paclitaxel concentrations ranging from 1 to 30 nM alone or with 5 mM metformin. MTT assay was performed to determine the level proliferation. (B) Genetic mouse model of ovarian cancer. A cancer cell line was established from the LSL-K-rasG12D/+PtenloxP/loxP genetic mouse model and proliferation was measured after treatment with paclitaxel alone or plus metformin. In a in vivo treatment study design, cancer was initiated in virginal LSL-K-rasG12D/+PtenloxP/loxP mice and two weeks later mice were treated with placebo (100 μl sterile PBS, n=21), paclitaxel alone (3 mg/kg/week injected i.p., n=25), metformin alone administered in drinking water (100 mg/kg/day, n=18) or paclitaxel plus metformin (n=29). Columns, fold change in tumor volume, normalized to control group; *, P=0.02. Dotted line, indicates 50% reduction in percent of proliferating cells.