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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Insect Biochem Mol Biol. 2015 Feb 17;59:58–71. doi: 10.1016/j.ibmb.2015.02.005

Figure 4.

Figure 4

Effect of MCO1 knock down on iron content in adult mosquitoes. Quantitative RT-PCR was used to determine the extent of knock down of AgMCO1 4 days (A) and 21 days (B) after injection of dsRNA. Knock down was apparent at 4 days but not 21 days post-injection. A Ferrozine-based assay was used to determine the iron content in control and AgMCO1 knock down mosquitoes. A decrease in iron content was observed for insects that were fed 10% sucrose (C) and for those that were fed 10% sucrose supplemented with 5 mM ferric ammonium citrate (D). Data were from three biological replicates. Means ± SD are shown. Differences in expression and in iron content were assessed by performing an unpaired t test. (*P < 0.05, ***P < 0.001, NS = no statistically significant difference.) (Abbreviations: C, control; KD, knock down.)