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. Author manuscript; available in PMC: 2016 Apr 1.

Published in final edited form as: Insect Biochem Mol Biol. 2015 Feb 17;59:58–71. doi: 10.1016/j.ibmb.2015.02.005

Table 5.

Kinetic characterization of the ferroxidase and ascorbate oxidase activity of DmMCO1 mutant isoforms.

	Ferrous iron			Ascorbate
DmMCO1T mutant isoform	K_cat (min^-1)	K_m (mM)	k_cat/K_m (min^-1 mM^-1)	k_cat (min^-1)	K_m (mM)	k_cat/K_m (min^-1 mM^-1)
[R454A]^a	42 ± 2.7	0.12 ± 0.02	359	1,480 ± 170	0.27 ± 0.05	5,516
[R454A, H374S]	35 ± 4.8	0.10 ± 0.03	350	294 ± 34	0.20 ± 0.06	1,487
[R454A, D380A]	54 ± 14	0.38 ± 0.15	139	243 ± 12	0.05 ± 0.01	4,470
[R454A, E552A]	48 ± 7.8	0.17 ± 0.05	276	747 ± 129	0.24 ± 0.06	3,135
[R454A, D380A, E552A]	39 ± 1.8	0.13 ± 0.01	295	765 ± 145	0.36 ± 0.14	2,104
[R454A, H374S, D380A, E552A]	40 ± 3.3	0.21 ± 0.03	191	no detectable activity

Note: the kinetic constants reported are best-fit values with standard errors

^a

Data are from Lang et al., 2012a