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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Free Radic Biol Med. 2015 Jan 21;82:137–146. doi: 10.1016/j.freeradbiomed.2015.01.007

Figure 2. HNE inhibits LKB1 and AMPK activities.

Figure 2

(A) LKB1 was immunoprecipitated from homogenates of cultured ARVMs incubated for 1 hour with vehicle or 10 μM HNE. Immunoprecipitated LKB1 was analyzed by immunoblotting for HNE adduct formation by densitometry and normalized to total LKB1 as a control for loading (n=6). (B) LKB1 activity in ARVMs treated with HNE (n=6–7). (C) AMPK activity in ARVMs treated with HNE (n=6–7). (D) Levels of phosphorylated ACC (P-ACC) were quantified by densitometry and normalized to total ACC (T-ACC) and GAPDH (n=3). For all of these experiments each replicate is an independent experiment conducted in duplicate using cells obtained from separate preparations of ARVMs, * = p < 0.05, and error bars are mean ± standard error.