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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Free Radic Biol Med. 2015 Jan 21;82:137–146. doi: 10.1016/j.freeradbiomed.2015.01.007

Figure 3. Hypertrophic mTOR-P70S6K and RPS6 phosphorylation are induced by HNE.

Figure 3

(A) Levels of phosphorylated mTOR (P-mTOR) were quantified by densitometry and normalized to total mTOR (T-mTOR) and GAPDH (n=3). (B) Levels of phosphorylated p70S6K (P-p70S6K) were quantified by densitometry and normalized to total p70S6K (T-p70S6K) and GAPDH (n=3). (C) Levels of phosphorylated RPS6 (P-RPS6) were quantified by densitometry and normalized to GAPDH (n=4). For all of these experiments each replicate is an independent experiment conducted in duplicate using cells obtained from separate preparations of ARVMs, * = p < 0.05, and error bars are mean ± standard error.