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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Free Radic Biol Med. 2015 Jan 21;82:137–146. doi: 10.1016/j.freeradbiomed.2015.01.007

Figure 4. HNE stimulates Erk1/2 in a MEK-dependent manner.

Figure 4

(A) Homogenates of cultured ARVMs incubated for 1 hour with vehicle or 10 μM HNE were evaluated for levels of phosphorylated Akt (P-Akt) by immunoblotting. Protein loading was normalized to total Akt (T-Akt) and GAPDH (n=3). (B) Levels of phosphorylated Erk1/2 (P-Erk) were quantified by densitometry and normalized to total Erk1/2 (T-Erk) and GAPDH (n=4). (C) ARVMs were incubated with vehicle or U0126 (10 μM, 30 minutes) prior to stimulation by vehicle or HNE. Lysates were immunoblotted for levels of P-Erk and normalized to T-Erk and GAPDH (n=4). For all of these experiments each replicate is an independent experiment using cells obtained from separate preparations of ARVMs, * = p < 0.05, ** = p < 0.01 and error bars are mean ± standard error.