ARVMs were treated with either a non-targeting control siRNA (Ctrl siRNA) or siRNA directed against LKB1 (LKB1 siRNA) for 72 hrs. After 72 hrs of siRNA exposure the ARVMs were treated with vehicle or HNE (10 μM, 1 hr) and the lysates were immunoblotted for protein expression of LKB1, P-RPS6, P-ACC, and GAPDH (n=3). For all of these experiments each replicate is an independent experiment using cells obtained from separate preparations of ARVMs, * = p < 0.05, ** = p < 0.01, *** = p < 0.001 and error bars are mean ± standard error.