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. Author manuscript; available in PMC: 2016 Apr 10.
Published in final edited form as: Exp Cell Res. 2015 Feb 13;333(1):39–48. doi: 10.1016/j.yexcr.2015.02.002

Figure 3. PiT-1 constructs transduced into PiT-1 ΔSM VSMCs are expressed and did not alter PiT-2 mRNA levels.

Figure 3

(A) Q-PCR quantification of PiT-1 mRNA expression of PiT-1 ΔSM VSMCs transduced with vector control, PiT-1 WT, PiT-1-E74K, PiT-1-S132A, or PiT-1-S623A confirmed stable expression. (B) Immunocytochemical analysis of PiT-1 ΔSM VSMCs expressing Vector Control, PiT-1-WT, PiT-1-E74K, PiT-1-S132A, or PiT-1-S623A with primary PiT-1 antibody (red) and DAPI counterstain (blue) show expression of the PiT-1 WT, PiT-1-E74K, PiT-1-S132A, and PiT-1-S623A proteins, scale bar is 25 µm. (C) PiT-2 mRNA quantification by Q-PCR confirmed no significant effects on PiT-2 mRNA expression in any cell line. Data presented as mean ± S.D. (A,C) or representative images (B).