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. Author manuscript; available in PMC: 2016 Apr 10.
Published in final edited form as: Exp Cell Res. 2015 Feb 13;333(1):39–48. doi: 10.1016/j.yexcr.2015.02.002

Figure 6. PiT-1 promotion of VSMC osteochondrogenic differentiation does not require Pi uptake.

Figure 6

(A) SM22α RNA expression in PiT-1 ΔSM VSMCs expressing vector control, PiT-1 WT, or PiT-1-E74K was quantified by Q-PCR after incubation in 1.0 mM Pi for 4 days. (B) OPN RNA expression is presented as fold-induction of 2.6 mM Pi over 1.0 mM Pi after 4 days of incubation. Data presented as mean ± S.D., n = 3 for all data points. Statistically significant differences of means compared to vector control are indicated by * = P<0.05 as measured by One-way ANOVA post-hoc Tukey analysis.