. Author manuscript; available in PMC: 2016 Apr 10.

Published in final edited form as: Exp Cell Res. 2015 Feb 13;333(1):39–48. doi: 10.1016/j.yexcr.2015.02.002

Table 1.

Site-directed mutagenesis primers and real-time Q-PCR primers and probes.

Function	Gene	Primer Sequence (5’ to 3’)	Accession Number
Site-directed mutagenesis	PiT-1-E74K	F: CTTAGCTAGCATCTTCAAAACTGTGGGCTCCGC	N/A
		R: GCGGAGCCCACAGTTTTGAAGATGCTAGCTAAG
	PiT-1-S132A	F: GCTTCGTTTTTGAAGCTTCCGATTGCTGGGACCCATTG
		R: CAATGGGTCCCAGCAATCGGAAGCTTCAAAAACGAAGC
	PiT-1-S623A	F: ACATTGGCCTTCCCATCGCCACAACACATTGCAAA
		R: TTTGCAATGTGTTGTGGCGATGGGAAGGCCAATGT

Q-PCR	OPN	F: TGAGGTCAAAGTCTAGGAGTTTCC	NM_009263
		R: TTAGACTCACCGCTCTTCATGTG
		P: *FAM–TTCTGATGAACAGTATCCTG–MGB*
	SM22α	F: GACTGACATGTTCCAGACTGTTGAC	NM_011526
		R: CAAACTGCCCAAAGCCATTAG
		P: *FAM–TGAAGGTAAGGATATGGCAGC–MGB*
	PiT-1	F:TTCCTTGTTCGTGCGTTCATC	NM_015747
		R:AATTGGTAAAGCTCGTAAGCCATT
		P:*FAM-CCGTAAGGCAGATCC-MGB*
	PiT-2	F:GACCGTGGAAACGCTAATGG	NM_011394
		R: CTCAGGAAGGACGCGATCAA
		P:*FAM-CATGGTTGGTTCAGCTG-MGB*

Primers and probes used in either site-directed mutagenesis or Q-PCR are listed here with the experimental function, the gene name, and the amplicon size and accession number if applicable. Primers used in site-directed mutagenesis have the desired mutation underlined. Q-PCR probes are FAM-reporters with MGB quenchers. F = Forward primer; R = Reverse primer; P = Probe.