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. Author manuscript; available in PMC: 2016 Jan 23.
Published in final edited form as: Oncogene. 2014 Oct 6;34(30):3908–3916. doi: 10.1038/onc.2014.321

Figure 2. TGFβ1 induced reversible EMT responses in A549 cells.

Figure 2

A549 cells were treated with TGFβ1 (2 ng/ml) for the indicated days (marked TGFβ1 treatment). After 12 days, TGFβ1-treated A549 cells were washed with PBS and subsequently cultured in normal growth medium without TGFβ1 for 3, 6, or 9 days. (a) Protein levels of key EMT functional proteins and metabolic enzymes were analyzed by western-blotting. (b) mRNA levels of ChREBP were monitored by RT-PCR. (c) mRNA levels of EMT functional proteins (E-cadherin, N-cadherin, and Snail1) and (d) lipogenic transcriptional regulatory factors (PPAR-gamma, SREBP1, and SREBP2) were monitored by RT-PCR. (*P<0.05 comparing to day0. #P<0.05 comparing to day12 TGFβ1 treatment.) (Data are represented as mean ± SEM.)