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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Exp Eye Res. 2014 Dec 19;131:42–55. doi: 10.1016/j.exer.2014.12.011

Figure 3. Lens epithelial cell lines exhibit gene expression pattern similar to isolated lens epithelium compared to lens fiber cells.

Figure 3

(A) Real time quantitative RT-PCR confirms significant up-regulation of lens epithelium-expressed genes Anxa2, Mcm4, and Pax6 in the LECs and isolated lens epithelium when compared to isolated lens fiber cells. (B) In contrast, real time quantitative RT-PCR exhibits significant down-regulation of lens fiber cell-expressed genes Crybb1, Mip, and Prox1 in the LECs and isolated lens epithelium when compared to isolated lens fiber cells. (C) Regular semi-quantitative RT-PCR demonstrates relative expression levels of the epithelium marker Foxe3, the fiber cell early differentiation marker Prox1 and the fiber cell differentiation marker Crybb1 in isolated lens epithelium and fiber cells as well as in 17EM15, 21EM15 and αTN4 cells. (D) Heat-map of comparative gene expression analysis between isolated lens epithelium and fiber cell microarrays and RNA-seq data and the LEC 21EM15 microarrays for select epithelium- or fiber cell-enriched genes. Asterisk denotes p-values < 0.05.